For one to awaken the Demon Slayer Mark, they must survive extremely life-threatening conditions, specifically a heart rate of over 200 beats per minute and a body temperature of over 39° Celsius (102.2° Fahrenheit), conditions which can prove to be fatal in most cases.  Muichiro that survival is the key difference that separates those that are marked and those who fail to awaken it. Also the people that awakened the Mark are going to die before reaching the age of 25

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